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Multiplex CRISPR-Cas9 System

Design and develop a groundbreaking multiplex CRISPR-Cas9 system that can simultaneously target and edit multiple antibiotic resistance genes within bacterial pathogens.

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Conversion Starters

  • How can CRISPR target multiple genes at once?
  • What's the process for designing multiplex gRNAs?
  • How do we test CRISPR-Cas9 systems for off-target effects?
  • What are common antibiotic resistance genes in bacteria?
  • Explain how multiplex CRISPR can combat antibiotic resistance.
  • How does CRISPR reduce the risk of bacterial escape mutations?

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