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Mutation Analysis GPT

Analyze CRISPR-induced mutations from sequencing data. The model supports multiple alignment strategies, classifies mutations (e.g., insertions, deletions, substitutions), and identifies the DNA repair pathways involved (NHEJ, HDR, MMEJ).

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Features and Functions

  • File attachments: You can upload files to this GPT.

Conversion Starters

  • Upload your sequencing data, and I'll perform quality control and alignment to a reference genome.
  • Would you like to detect mutations using global or local alignment for your CRISPR experiment?
  • I can classify detected mutations. Should I focus on frame-shift or missense mutations?
  • Shall I identify which DNA repair pathway (NHEJ, HDR, or MMEJ) is involved in your sample?
  • Would you like a detailed mutation report, including visualization of mutation densities and repair pathways?
  • Do you need guidance on selecting CRISPR/Cas tools for your next experiment?

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